DNA

Part:BBa_K4051024:Design

Designed by: Clodagh Healy   Group: iGEM21_MTU-CORK   (2021-10-11)


PCRC GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 363
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 728
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 718
    Illegal SapI.rc site found at 245


Design Notes

Codon optimised for E.coli


Source

Generic histag and secretion signal Dechloromonas (Azospira) suillum locus Dsui_0147

Genbank Accession: CP003153.1


References